Requisition Form for Mouse Line Cryopreservation, Cryo-recovery, IVF and rederivation

If you desire more than one strain, please complete a separate form for each of them

Description of requested procedure

Requested Procedure *

Cryopreservation Sperm + IVF test

Cryo-recovery

Embryo Cryo-recovery

Genotype

Live stock rediveration

Only Cryo preservation Sperm / Oocytes / Embryo

Cryo recovery Oocytes

Sperm Cryo-recovery +IVF

Storage Cryo Import

Live stock rederivation

To generate pathogen free mouse lines, embryo is isolated from donor female and transferred to a clean recipient pseudo-pregnant female.

Quantity and names of target genes *

Please indicate where donor animals are currently being housed / will be coming from *

Known pathogens to be cleared out to meet SPF1 standards? *

Genetic background of embryos to be rederived *

In case of multigenic/compound lines to be rederived: what is the estimated % of progeny of each potential genotype to be recovered? *

Has the oocyte female donor line been tested for superovulation efficiency? *

Yes

N/A

Desired breeding scheme upon stock rederivation *

Cryo-recovery

Frozen sperm will be resuscitated by IVF and embryo transfer. Frozen embryos will be recovered and transplanted into pseudopregnant recipients. Sperm or embryos can be received frozen from other institutes.

Cryo Sample IDs *

Number of cryo samples being provided *

Cryo-recovery of stocks preserved by external facilities *

Please attach the detailed protocol that was used for cryopreservation and recommended cryo-recvery method
More information

Files must be less than 2 MB.
Allowed file types: gif jpg jpeg png txt rtf html pdf doc docx ppt pptx xls xlsx.

Embryo Cryo-recovery

Please indicate origin of cryo-stocks and current repository name *

Sperm Cryo-recovery +IVF

Please indicate the genetic background of oocyte donor female to be used *

Storage Cryo Import

You have chosen cryo preservation only, without an IVF recovery test. *

Please confirm that you take full responsibility for all future cryo preservation recovery outcomes.

Cryopreservation of either sperm or embryos enables you to save your colony in case of contamination and dramatically reduce costs associated with mouse colony maintenance, particularly of low use strains

Strain Name *

Name as will be printed on the straws, maximum 16 characters

Background Strain *

Quantity and names of target genes *

Do you have a routine PCR (or other means) to check the required strain? *

Please indicate the genetic background of oocyte donor female to be used. *

Name of submitter *

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